conditioned stimulus cst Search Results


anti e cadherin  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti e cadherin
(A) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 5 min – 72 h and analyzed for pSTAT3 Y705 , tSTAT3, SNAIL, TWIST and <t>E-CADHERIN</t> levels by immunoblotting. β-ACTIN and HSC-70 were used as loading controls. ( B ) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 1 h and examined for mRNA levels of E-Cadherin , SNAIL , SLUG and TWIST by real-time qRT-PCR ( * p<0.05, ** p<0.01, n=3 experiments, Student’s t-test). (C) FACS histogram plots (blue: ctrl treated, red: opioid-treated 1 μM, 1 h) of MCF-7 and MDA-MB-231 cells stained against E-Cadherin. Dotted curves represent FACS histogram plots for the isotype-matched controls. (D) Representative H&E, Cleaved Caspase-3 and Vimentin stained lung sections of mice engrafted with MDA-MB-231 cells after primary tumor removal and post-surgical opioid treatment for ten days (original magnification: 40x and 100x; scale bars 200 μm and inserts 100 μm).
Anti E Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conditioned+stimulus+cst/E-Cadherin+Rabbit+mAb/bio_rxiv__443663-126-21-24
Average 95 stars, based on 1 article reviews
anti e cadherin - by Bioz Stars, 2026-09
95/100 stars
  Buy from Supplier
rabbit mab cst rab7 d95f2 rabbit mab rab5  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit mab cst rab7 d95f2 rabbit mab rab5
(A) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 5 min – 72 h and analyzed for pSTAT3 Y705 , tSTAT3, SNAIL, TWIST and <t>E-CADHERIN</t> levels by immunoblotting. β-ACTIN and HSC-70 were used as loading controls. ( B ) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 1 h and examined for mRNA levels of E-Cadherin , SNAIL , SLUG and TWIST by real-time qRT-PCR ( * p<0.05, ** p<0.01, n=3 experiments, Student’s t-test). (C) FACS histogram plots (blue: ctrl treated, red: opioid-treated 1 μM, 1 h) of MCF-7 and MDA-MB-231 cells stained against E-Cadherin. Dotted curves represent FACS histogram plots for the isotype-matched controls. (D) Representative H&E, Cleaved Caspase-3 and Vimentin stained lung sections of mice engrafted with MDA-MB-231 cells after primary tumor removal and post-surgical opioid treatment for ten days (original magnification: 40x and 100x; scale bars 200 μm and inserts 100 μm).
Rabbit Mab Cst Rab7 D95f2 Rabbit Mab Rab5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/conditioned+stimulus+cst/Rab5+Rabbit+mAb/pm38941888-43-162-164
Average 96 stars, based on 1 article reviews
rabbit mab cst rab7 d95f2 rabbit mab rab5 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


(A) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 5 min – 72 h and analyzed for pSTAT3 Y705 , tSTAT3, SNAIL, TWIST and E-CADHERIN levels by immunoblotting. β-ACTIN and HSC-70 were used as loading controls. ( B ) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 1 h and examined for mRNA levels of E-Cadherin , SNAIL , SLUG and TWIST by real-time qRT-PCR ( * p<0.05, ** p<0.01, n=3 experiments, Student’s t-test). (C) FACS histogram plots (blue: ctrl treated, red: opioid-treated 1 μM, 1 h) of MCF-7 and MDA-MB-231 cells stained against E-Cadherin. Dotted curves represent FACS histogram plots for the isotype-matched controls. (D) Representative H&E, Cleaved Caspase-3 and Vimentin stained lung sections of mice engrafted with MDA-MB-231 cells after primary tumor removal and post-surgical opioid treatment for ten days (original magnification: 40x and 100x; scale bars 200 μm and inserts 100 μm).

Journal: bioRxiv

Article Title: Opioids trigger breast cancer metastasis through E-Cadherin downregulation and STAT3 activation promoting epithelial-mesenchymal transition

doi: 10.1101/443663

Figure Lengend Snippet: (A) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 5 min – 72 h and analyzed for pSTAT3 Y705 , tSTAT3, SNAIL, TWIST and E-CADHERIN levels by immunoblotting. β-ACTIN and HSC-70 were used as loading controls. ( B ) MDA-MB-231 and MCF-7 cells were treated with 1 μM opioid for 1 h and examined for mRNA levels of E-Cadherin , SNAIL , SLUG and TWIST by real-time qRT-PCR ( * p<0.05, ** p<0.01, n=3 experiments, Student’s t-test). (C) FACS histogram plots (blue: ctrl treated, red: opioid-treated 1 μM, 1 h) of MCF-7 and MDA-MB-231 cells stained against E-Cadherin. Dotted curves represent FACS histogram plots for the isotype-matched controls. (D) Representative H&E, Cleaved Caspase-3 and Vimentin stained lung sections of mice engrafted with MDA-MB-231 cells after primary tumor removal and post-surgical opioid treatment for ten days (original magnification: 40x and 100x; scale bars 200 μm and inserts 100 μm).

Article Snippet: To assess E-Cadherin cell surface expression, cells were stimulated for 1 h with 1 μM of the opioid following incubation with anti-E-Cadherin (dilution 1:1000, CST, 3199).

Techniques: Western Blot, Quantitative RT-PCR, Staining